WST-1 Cell Proliferation Assay Kit

Easy – No cell washing, harvesting or solubilization steps required
Faster and more sensitive than MTT/XTT or MTS-based assays
Can be used to perform real-time, continuous, live cell viabilitiy assays.

The Quick Cell Proliferation Kit is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion of viable cell numbers results in an increase in the overall activity of the mitochondrial dehydrogenases in the sample, corresponding to an increase in formazan dye metabolism. The formazan dye produced by the viable cells is measured at an absorbance of 440 nm using a standard multiwell spectrophotometer. The assay is used to determine cell proliferation in response to interactions with drugs, growth factors, cytokines, mitogens, and nutrients. This simple method does not require cell washing, harvesting, or any solubilization steps.

Kit Components

WST-1 Reagent (lyophilized)
Electro Coupling Solution (ECS)

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WST-1 Cell Proliferation Assay Kit

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