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Membrane-Based Cell Culture

We support cell culture with a large family of new and innovative membrane-based products and protocols. Our Millicell plates offer numerous technical advantages over traditional, solid-bottom plastic plates, especially for epithelial and endothelial cells. With solid bottom plastic tissue culture plates, cells can only access media from their apical side. Millicell microporous inserts nurture cells in a three dimensional environment, resulting in cell morphology that mimics in vivo growth.

Our products range from single well standing and hanging inserts to 24 and 96-well cell culture systems for greater flexibility in the lab. You can also choose from numerous membrane types, from those that offer low protein binding to others that allow cell attachment without ECM coating.

We provide protocols for monolayer integrity and drug transport, organotypic work, co-culture stem cells and in vitro toxicology, offering you complete solutions for your cell culture research needs.

Microporous Membrane-based Cell Culture

Millicell Cell Culture Inserts promote natural cell growth and incorporate unique design features to improve flexibility in today’s laboratories....
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Application Guide

Application Guide and filter code pore size recommendations...
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Single-well Cell Culture Inserts

Individual Millicell cell culture inserts provide users flexibility in the number of samples to run at one time...
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Multi-well Cell Culture Insert Plates

Millicell 24- and 96-well cell culture insert plates are designed to support cell attachment, growth and differentiation for many cell lines including MDCK and Caco-2...
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Co-culture in Millicell Cell Culture Inserts

Co-culturing procedures involve growing two or more cell types in culture simultaneously. Cell types can be co-cultured in direct contact with each other on the membrane or indirectly...
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In vitro Toxicology

In vitro models play an important role in the toxicity testing of cosmetics, drugs and pharmaceutical therapies. These assays are being used earlier in toxicity testing pipelines...
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Automation

Millicell-24 and Millicell-96 cell culture insert plates are ideal for use in high throughput cell culture labs. These streamlined plate designs enhance compatibility with robotic seed-and-feed systems...
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Cell Culture Equipment & Supplies

Millipore’s popular range of pipette holders are designed by cell culturists to enable convenient access to pipettes...
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Recommended Working Volumes

Single-well, 24-well and 96-well inserts are available to meet a range of application needs. The protocols printed in this section of the guide can be adjusted to accommodate a range of platforms...
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Seeding and Feeding Guidelines

Optimizing cell seeding protocols and densities is extremely important to ensure good cell attachment and growth in Millicell-24 cell culture insert plates...
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ECM Coating

Coating of membranes and plastic surfaces with extra cellular matrices (ECM) promotes cell attachment and monolayer formation. The following protocols describe coating with four types of ECM on Millicell-CM inserts...
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Monolayer Integrity and Drug Transport Guidelines

Millicell-24 cell culture insert plate is a 24-well general purpose device designed to support cell growth, attachment, differentiation, or other desired applications. The procedure described below details how to measure the formation of a differentiated cell monolayer...
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Organotypic Cell Culture

Millicell-CM organotypic inserts are sterile, single-use, 30 mm devices designed for customers who need to do long-term organotypic culture. ..
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Co-Culture in Millicell Cell Culture Inserts Protocol

Step-by-step protocol...
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Fixation and Staining

Millicell cell culture inserts (single-well, 24-well plates and 96-well plates) are designed to support all fixation, staining and immunostaining procedures in a single device...
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Visualization and Microscopy

Sep-by-step protocol...
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Sodium Fluorescein Permeability Assay

sStep-by-step protocol...
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Pathways