Gene Targeting
Mammalian Cell Transfection Kit
The Mammalian Cell Transfection Kit is an optimized system suitable for the introduction of DNA into mammalian cells. DNA is applied to the cells as a calcium phosphate precipitate. Transfected cells integrate and express the exogenous DNA at a higher efficiency, producing stable clones of the specifically altered genotype and phenotype. This system has been used to introduce various plasmids into a broad range of cell lines.
Transient Expression Transfection Kit
The Transient Expression Transfection Kit contains all of the necessary reagents to transfect DNA into mammalian cells as a DEAE Dextran-DNA complex.The DEAE dextran mediated transfection method is used for highly efficient transient expression of cloned genes. This method has been shown to be most effective in COS-7, COS-1 and CV-1 cells; however, a large number of additional lines have been successfully transfected using this method. These include: L-cells, GH-3, Vero, CHO and NIH 3T3 cells. Transfection of these other cell types requires modification of the protocol with respect to the time of incubation of cells with DEAE dextran-DNA-media and chloroquine.
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Pathways
