Fast-Trap™
Purification
and Concentration Kit
How would your laboratory workflow change if you could purify and concentrate your virus ALL IN ONE DAY?
The Fast-Trap Virus Purification and Concentration kits can prep your virus work in record time!
Highly purified viruses are essential for applications, such as vaccine production and genetic modification of cells. Conventional virus purification methods based on sucrose or cesium chloride gradient ultracentrifugation are time-consuming, difficult, and require expensive instrumentation. Likewise, most membrane-based purification methods involve messy and potentially hazardous steps. These protocols can also be hampered by low virus recovery. In response to customer requests, Millipore has created two new Fast-Trap virus purification kits that bypass the problems of traditional methods.The Fast-Trap Adenovirus and Lentivirus Purification and Concentration Kits are efficient tools for virus preparation. Unlike traditional cumbersome virus purification methods with cesium chloride that can take days, the kits are designed to help you cut your virus prep work down to two hours! The kits use Millipore’s innovative vacuum-driven Steriflip device containing a new virus membrane to purify crude virus samples, followed by a concentration step using Amicon Ultra spin filter. Fast-Trap kits will give you high recoveries of purified virus in less time using a simple protocol.
Fast-Trap Advantages
- Saves Time: protocol can be completed in as little as two hours
- Higher Yield: recover up to 70% of viral particles
- Dependable: simple protocol ensures quality results
- Easy: Steriflip filters eliminate messy purification steps and expensive equipment
- Safe: close vacuum device eliminate potential spills
Millipore’s Fast-Trap Adenovirus and Lentivirus Purification and Concentration Kits provide a fast, safe, and easy alternative for viral purification. The kits contain the necessary components to accommodate the entire virus purification workflow. The purification results in high recovery of viable viral particles with good purity.
FASTER
Millipore's new Fast-Trap Kit was compared with other common methods to determine which methods gave the best recoveries in the least time. The Fast-Trap Kit was faster than the other methods while giving similar recovery.
| Kit | Format | Total
Processing Time | Comments |
| Millipore Fast-Trap Kit | Vacuum | 21 min | Millipore Fast-Trap Virus Purification and Concentration Kits are fast, safe, easy and dependable |
| S | Syringe filter | 40 min | Flow rate difficult to control, left with “messy” impression due to occasional drop of liquid when changing syringe filters |
| V | Centrifugal | 1 h 20 min (40 min if no clogging) | Clarification device clogged |
| Traditional CsCI | CsCI Density Gradient | Approx. 48 hrs | Labor intensive and requires specialized laboratory equipment |
HIGHER RECOVERY
The Fast-Trap kits, when compared with traditional cesium chloride and sucrose gradient purification methods, demonstrate a significant increase in viral recovery.
 | ||
| % Adenovirus Recovery | 70 | 40 |
| ||
| % Lentivirus Recovery | 47 | 22 |
Below: Virus purification workflow using the Fast-Trap Adenovirus (Cat. No. FTAV00003) and Lentivirus (Cat. No. FTLV00003) Purification and Concentration Kits.
Concentration Kits | |
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