Disassociation Reagents
Now researchers can tailor their passaging and culturing requirements to their experimental needs with our extensive line of enzyme and non-enzymatic formulations for cell dissociation. Everything from traditional trypsin solutions to our Accutase™ and Accumax™ solutions that provide researchers with gentle and effective, non-mammalian derived solutions for cell detachment, are now available. Our unique cell culture freezing media formulations (DMSO and Glycerol formats) are ideal for the cryopreservation of a broad spectrum of mammalian cells. These products result in consistent cryopreservation and high cell viability upon thawing and plating. The components used in this product line are certified to be virus and mycoplasma free, supplied sterile and come with a complete protocol for the freezing and thawing of your cells.
Accutase Solution
An unique cell detachment solution of proteolytic and collagenolytic enzymes for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware. Accutase does not contain mammalian or bacterial derived products. Accutase has been shown to be effective on a wide variety of cell types including: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3,Vero, OS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells and Sf9 insect cells.Benefits
- Detaches adherent cells in minutes
- Gentle cell detachment for maximum cell viability
- Highest plating efficiency
- Cost effective and ready-to-use
Human MG63 fibrosarcoma cells cultured on tissue culture treated dishes in DMEM +10% FBS were treated with Accutase. Treatment results in rapid cell detachment, a single cell suspension and high viability. Accutase is gentle on cells; viability was 97% ± 3% even after 45 minutes in Accutase. | |
Various constructs of genetically engineered CHO cells, BHK cells and a hybridoma were grown in suspension in serum-free or protein-free medium. Representative cell aliquots were treated with an equal volume of PBS or Accumax and incubated for 5 minutes at 37°C. Cell number was then determined with a Coulter Counter. |
Accumax
A proprietary cell detachment solution of proteolytic, collagenolytic and DNAse enzymes. Useful for creating single cell suspensions from clumped cell cultures for accurate cell counting and detachment of cells from primary tissue. Accumax does not contain mammalian or bacterial derived products.Benefits
- Dissociates clumped cells in minutes
- Results in single cell suspensions
- Yields accurate, reproducible cell counts
- Dissociates tissues for primary cell culture
Enzyme Free Cell Dissociation Solutions
Our unique non-enzymatic solutions, which contain no protein or surfactants, are composed of chelating agents and other agents used to stabilize their activity on the cells. These reagents gently dislodge adherent cells from their substrates, while preserving the structural and functional integrity of cell surface proteins. There are no cytotoxic effects associated with these solutions, such as are sometimes associated with the use of 0.5 mM EDTA. They can be used to dissociate primary cells, tissues and tumors, while allowing for increased efficiency.
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