• Login
|
• Register
|
• My Account
|
• Shopping Cart
|
• Help

Chromatin IP

The Nexus of Genomics & Proteomics

Chromatin Immunoprecipitation: the Nexus of Genomics and Proteomics

Critical to understanding the biological function of a particular histone modification or chromosomal protein is the identification of its distribution throughout the genome and how that distribution is altered as part of specific biological processes. Currently, the chromatin immunoprecipitation (ChIP) technique is the most precise method to identify specific proteins associated with a region of the genome or, conversely, identification of regions of the genome associated with specific proteins. The proteins in question might be modified isoforms of histones or non-histone chromosomal proteins. Thus, one can use ChIP to quantitate the relative amount of histone H3 acetylation, for example, that is associated with a specific gene promoter under various conditions that alter expression of the gene. However, much of the recent usage of ChIP has been for identifying and analyzing transcription factor binding sites.

Schematic representation of the chromatin immunoprecipitation (ChIP) technique. First, the proteins are cross-linked to DNA with formaldehyde, and then the chromatin is sheared to a manageable size by sonication. Specific proteins are immunoprecipitated with antibodies, also bringing down the DNA to which the protein is cross-linked. The cross-links are reversed, the DNA purified, and the sample is interrogated for the enrichment of specific DNA sequences. The detection step can be performed most accurately by quantitative real-time PCR, but the usage of microarrays in this step is increasing.

The information contained within chromatin modifications has great promise to advance our understanding and treatment of diseases such as cancer. Consider the case in which a gene is either a tumor suppressor inactivated by an epigenetic mechanism or an inappropriately activated oncogene. Using the ChIP technique, it is possible to identify the histone modifications (and transcription factors) associated with this gene and consequently the enzymes that have deposited the ectopic marks. Thus, targets for therapeutic intervention emerge from easily obtained epigenetic information.

New techniques that combine the ChIP technique with microarray analysis facilitate the determination of genome-wide distribution patterns for chromosomal proteins and histone modifications. Using this combined technique, researchers can ask sweeping questions regarding the cellular functions of histone modifications and transcription factors and place these in the larger context of regulatory network interactions.

back to top

// this function fires when the user has clicked on a tab var onTabClicked = function(tabIndex, obj) { window.location.href = getBaseURL() + "#" + tabIndex; AntibodiesNavigatorPanels.setSelectedIndex(tabIndex); } mainSimpleTabs=new SimpleTabs("find_antibodies", 0, onTabClicked); AntibodiesNavigatorPanels.addNavigatorPanel(new NavigatorPanel("primary_antibodies_form", "primary_antibodies_filter", "/pages/microsites/antibodies/primary_antibodies_filter.jsp?navigatorPanelSet=AntibodiesNavigatorPanels", true)); AntibodiesNavigatorPanels.addNavigatorPanel(new NavigatorPanel("secondary_antibodies_form", "secondary_antibodies_filter", "/pages/microsites/antibodies/secondary_antibodies_filter.jsp?navigatorPanelSet=AntibodiesNavigatorPanels")); AntibodiesNavigatorPanels.setSelectedIndex(getTabIndex()); AntibodiesNavigatorPanels.populateFilters(); mainSimpleTabs.setTab(getTabIndex()); if (disableToggle) { $("toggleAdv").style.display = "none"; } AntibodiesNavigatorPanels.getNavigatorPanel(0).setToggleImageId("toggleAdv"); AntibodiesNavigatorPanels.setShowAll(true);

• Primary
• Secondary

Find Primary Antibodies

Enter a keyword and/or search criteria below. Or you may also use selections below to browse specific antibodies for your needs.

Keyword Gene Symbol UniProt Number Entrez Gene Number

Advanced Options

---

Find Secondary Antibodies

Select the host, conjugate, and species, and then press Search to find the secondary antibodies for your research needs.

Product News & Releases

• Faster Flow Rates for Sterile Filtration - New Millex® syringe filters with Millipore Express PLUS (PES) membrane
• ReNcell® VM Neural Stem Cell Line Models Parkinson’s Disease - New human disease model shows function of PINK1 gene in neural degeneration
• Millipore Issues New Laboratory Filtration Product Guide
• Millipore Launches Progenitor Cell-Enriched Primary Human Epithelial Cell Cultures

Featured Products

• Fast-Trap Lentivirus Purification and Concentration Kit
• Fast-Trap Adenovirus Purification and Concentration Kit
• EasyCyte Plus Flow Cytometry System
• ECM Cell Culture Optimization Arrays
• ESGRO Complete
• GCTM-5 Antibody, clone GCTM-5
• Guava Viacount Assay
• MilliTrace Constitutive GFP Reporter
• MultiScreen_HTS + and MultiScreen Vacuum Manifolds
• ENStem-A hES-derived Human Neural Progenitors