Cell Analysis

Cell analysis techniques improve our basic understanding of cellular mechanisms and help identify the proteins and pathways involved in disease.

We provide you one of the broadest offerings of multiwell filter plates, antibodies, and other reagents for studying cells.

In the section, you will find an overview of our products for immunocytochemistry/immunohistochemistry, western blotting, and immunoprecipitation. You will also find protocols for the detection of BrdU and free phosphates, as well as protocols for ELISA assays and cell staining. These procedures represent only a handful of the many techniques that we support.

Western Blotting

Since its introduction in 1979 (Towbin et al., 1979), protein blotting has become a routine tool in research laboratories. It is traditionally used to detect low amounts of proteins in complex samples or to monitor protein expression and purification.
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Immunoprecipitation

It is possible to use antibody-antigen precipitation (immuoprecipitation) to isolate a specific antigen from complex protein mixtures such as cell or tissue lysates. Immunoprecipitation has proven to be an invaluable investigational tool that is routinely employed by many laboratories to ascertain critical information regarding a given antigen. These include small scale antigen purification for functional studies, N-terminal sequence analysis, investigation of protein-protein interactions, and the determination of the relative abundance and stoichiometric distribution of the antigen within a cell or tissue, among other things.
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Immunostaining (ICC/IHC)

Immunohistochemistry/immunocytochemistry is the demonstration of a tissue or cellular constituent in situ by detecting specific antibody-antigen interactions where the antibody has been tagged with a visible label. The visual marker may be a fluorescent dye, colloidal metal, hapten, radioactive marker or an enzyme.
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Immunocytochemistry General Protocol

Immunocytochemistry (ICC) by definition is the demonstration of a tissue constituent in situ by detecting specific antibody-antigen interactions where the antibody has been tagged with a visible label. The visual marker may be a fluorescent dye, colloidal metal, hapten, radioactive marker or more commonly, an enzyme for light microscopy.
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BrdU Labeling & Immunocytochemistry Protocol

Adherent or non-adherent cells can be labeled. Usually the labeling is done for a short period, such as two hours as shown in this example. However one can label overnight or even days depending upon the experiment.
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Flow Cytometry

Flow Cytometry is a powerful multi-parametric single cell analysis platform with wide applications in basic research and clinical medicine for the diagnosis, isolation and study of specific cell types and disease conditions.
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ELISA Procedures

Enzyme-linked Immunosorbent Assays (ELISAs) combine the specificity of antibodies with the sensitivity of simple enzyme assays, by using antibodies or antigens coupled to an easily assayed enzyme that possesses a high turnover number. ELISAs can provide a useful measurement of antigen or antibody concentration.
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General Protocol for the Sandwich ELISA Method

Step-by-step procedures...
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General Protocol for the Competitive ELISA Method

Step-by-step procedures...
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Elispot

MultiScreenHTS filter plates are available with validated Immobilon®-P (PVDF) membrane for superior results. Plates with HA (mixed cellulose esters) are also available. Both provide membranes with dense uniform pore structure to promote antibody binding and increase sensitivity for better, sharper spot definition.
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Elispot Protocols

The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications.
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Cell Lysates and Tissue Extracts for Malachite Green Protocol

Cell lysates and tissue extracts contain millimolar concentrations of free phosphate that will interfere with the phosphatase assay. In addition, ATP at high concentrations can increase the background and lead to phosphorylation by contaminating kinases. It is therefore necessary to eliminate these components from samples by running samples over a Sephadex® G-25 resin (GE Healthcare Biosciences).
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