Cancer
In Vitro Vascular Permeability Assay
- Highly effective for evaluating effects of chemicals and drugs on endothelial cell absorption, transport and permeability
- Insert membranes contain a high density of 1.0 µm diameter pores, ideal for measuring compounds that disrupt the endothelial cell layer
- Sensitive fluorescent detection system
A fundamental requirement for the physiological performance of tissues and organs is the formation of diffusion barriers that separate and maintain compartments of different structure. Disruptions of the barrier integrity are manifested as microvascular hyperpermeability which is associated with many systemic disease states including: heart disease, diabetes, cancer, stroke, hypertension, arthritis, and Alzheimer’s. One example is the endothelial cell lining of the internal vasculature that defines a semi-permeable barrier between the blood and the interstitial spaces of the body. The In Vitro Vascular Permeability Assay provides an efficient system for evaluating the effects of chemicals and drugs on endothelial cell adsorption, transport and permeability.
With this assay kit, endothelial cells are seeded onto collagen-coated inserts and treated with cytokines, growth factors, or reagent of interest. After treatment, FITC-Dextran is added on top of the cells, allowing it to permeate through the cell monolayer. The extent of permeability is determined by measuring the fluorescence of the plate well solution.
Kit Components
- Permeability chambers: two 24-well plates with 12 cell culture inserts
- Permeability 24-well detection plate
- Collagen solution
- FITC-Dextran solution
- Forceps
HUVEC Monolayer Permeability HUVEC cells were seeded at 200,000 cells per insert and cultured for 72 hours. HUVEC monolayer permeability was tested after a 24 hour starvation period. Test inserts were then treated for 18 hours with 100 ng/mL of Interleukin-1â in basal medium. Monolayers were also treated with basal medium and growth medium only. Inserts were also tested without cell monolayer. The fluorescence of the plate well solution was determined using a standard fluorescent plate reader. |
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