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Actin /Cytoskeleton and Focal Adhesion Staining Kit

The actin cytoskeleton is a highly dynamic network composed of actin polymers and a large variety of associated proteins. The actin cytoskeleton mediates a variety of essential biological functions including intra- and extra-cellular movement and structural support. To perform these functions, the organization of the actin cytoskeleton must be tightly regulated both temporally and spatially.

Focal adhesion and adherens junctions are membrane-associated complexes that serve as nucleation sites for actin filaments and as cross-linkers between the cell exterior, plasma membrane and actin cytoskeleton. The function of focal adhesions are structural, linking the ECM on the outside of the cell to the actin cytoskeleton on the inside. They are also sites of signal transduction, initiating signaling pathways in response to adhesion.

Millipore’s Actin Cytoskeleton and Focal Adhesion Staining Kit is a sensitive immunocytochemical tool that contains three key components to monitor the regulation of cytoskeleton assembly. These are: (1) fluorescent- labeled phalloidin (TRITC-conjugated) to map the local orientation of actin filaments within the cell; (2) Vinculin monoclonal antibody to stain the focal contacts in cells; and (3) DAPI to stain the nucleus.

Confocal fluorescence microscopy of focal adhesion and actin cytoskeleton in COS-7 cells revealed with triple labeling using TRITC-conjugated phalloidin, anti-Vinculin and DAPI.

Kit Components

• Vinculin Monoclonal Antibody
• TRITC-conjugated Phalloidin
• DAPI

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