Immobilon Transfer Membranes, Sandwiches, and Blotting Filter Paper

Immobilon Transfer Membranes, Sandwiches, and Blotting Filter Paper Polyvinylidene fluoride (PVDF) for superior western, dot, and slot blots Millipore offers three different Immobilon PVDF membranes, each optimized for different protein blotting applications. We also now offer blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper. Immobilon membranes provide a number of advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Membrane
- 0.45 µm pore size
- Recommended for most western blotting, especially proteins >20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity


Blotting Sandwiches
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper.


Blotting Filter Paper
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.


Immobilon-P^SQ Membrane
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins <20 kDa
- Prevents blow-through of low molecular weight proteins


Immobilon-FL Membrane
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications


Performance
Rapid Immunodetection Protocol with Immobilon-P Membrane

Step Standard Immunodetection Rapid Immunodetection 1. Block the membrane 1 h None 2. Incubate with primary antibody 1 h 1 h 3. Wash the membrane 3 x 10 min 3 x 5 min 4. Incubate with secondary antibody 1 h 30 min 5. Wash the membrane 3 x 10 min 3 x 5 min 6. Add substrate 5 min 5 min Total time 4 h 5 min 2 h 5 min

Immobilon-P^SQ Membrane Prevents Blow-through

Molecular weight standards (lanes 1, 3, 5, 7) and calf liver lysate (lanes 2, 4, 6, 8) were transferred to Immobilon-P or Immobilon-PSQ membranes. A sheet of Immobilon-PSQ was placed behind the primary membranes to capture proteins that passed through (lanes 5 and 6 behind Immobilon-P; lanes 7 and 8 behind Immobilon-PSQ).

Immobilon-FL Membrane is Optimized for Fluorescence

Actin-tubulin multiplex detection on Immobilon-FL membrane. Rabbit muscle actin (red) was detected using rabbit anti-actin 1oAB and QDot® 655 goat anti-rabbit 2oAB. Porcine brain tubulin (green) was detected using mouse anti-tubulin 1oAB and QDot 565 goat anti-mouse 2oAB. Sensitivities down to 7 ng were observed for both analytes. Data provided by Quantum Dot Corporation.

Specifications    (return to top)

Description Immobilon-P The original PVDF membrane for western, dot, and other protein blots Immobilon‑PSQ Small pore structure results in superior binding of proteins with MW <20 kDa Immobilon-FL Optimized for fluororescent immunodetection applications Applications Immobilon-P Western blotting, binding assays, amino acid analysis, N‑terminal protein sequencing, dot/slot blotting, glycoprotein visualization, lipopolysaccharide analysis Immobilon‑PSQ Low-molecular-weight western blotting, amino acid analysis, N‑terminal protein sequencing Immobilon-FL Western blotting, dot/slot blotting Immobilon Blotting Filter Paper Western blotting, dot/slot blotting Compatible Stains/Dyes Immobilon-P Coomassie brilliant blue, amido black, India ink, Ponceau‑S red, colloidal gold, CPTS, toluidine blue, transillumination, Sypro® ruby Immobilon‑PSQ Coomassie brilliant blue, amido black, India ink, Ponceau‑S red, colloidal gold, CPTS, toluidine blue, transillumination, Sypro® ruby Immobilon-FL All fluorescence dyes, Coomassie brilliant blue dye, amido black, Ponceau‑S red, CPTS, Sypro® ruby

Immobilon-P Immobilon-PSQ Immobilon-FL Filter Material Hydrophobic PVDF Hydrophobic PVDF Hydrophobic PVDF Filter Pore Size, µm 0.45 0.2 0.45 Protein Binding Capacity, µg/cm2 Insulin 160 262 155 BSA 215 340 205 Goat IgG 294 448 300