Ordering Information
Product Images
Mouse anti-NG2 (Catalog Number MAB5384). Localization of NG2 in adult rat cortex.
Anti-NG2 Chondroitin Sulfate Proteoglycan
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ELISA, WB, IH | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May reg
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Entrez Gene Number:
Entrez Gene Summary
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Description:
Anti-NG2 Chondroitin Sulfate Proteoglycan
Background Information:
NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.
Clone:
132.39
Specificity:
Recognizes NG2 Chondroitin Sulfate Proteoglycan. MAB5384 reacts with native, recombinant and protein from cells which express NG2.
Key Applications:
- ELISA
- Western Blotting
- Immunohistochemistry
Application Notes:
Western blot. Recognizes the >280 kD NG2 protein. Suggested blocking buffer is 5% dry milk in TBST. Immunocytochemistry: 1:200-1:500 on oligodendrocyte precursor cells.
Immunohistochemistry. It is suggested that the tissue used is only lightly fixed (4% paraformaldehyde for less than 2 hours, etc.). Avoid overfixing tissue. Suggested blocking buffer is 2-4% normal serum. Suggested antibody dilution buffer is PBS containing 0.1% Triton X100.ELISA (direct)
Optimal working dilutions must be determined by end user.
Immunohistochemistry. It is suggested that the tissue used is only lightly fixed (4% paraformaldehyde for less than 2 hours, etc.). Avoid overfixing tissue. Suggested blocking buffer is 2-4% normal serum. Suggested antibody dilution buffer is PBS containing 0.1% Triton X100.ELISA (direct)
Optimal working dilutions must be determined by end user.
Species Reactivity:
Rat
Isotype:
IgG1
Immunogen:
Cell line expressing a truncated form of NG2.
Molecular Weight:
~ 280 kDa
Control:
Neonatal rat brain extract
Presentation:
Protein G Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, containing 0.25M NaCl and 0.1% azide.
Storage Conditions:
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- CSPG4
- MSK16
- MCSP
- NG2
- MCSPG
- MEL-CSPG
- HMW-MAA
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Concentration:
1 mg/mL
Format Code:
Pur
Trade Name:
Chemicon (Millipore)
Purification Method:
Protein G Purified
Format:
Purified
Antibody Type:
Monoclonal Antibody
Host:
Mouse