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Anti-Actin, clone C4
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ELISA, WB, IC, IH, IH(P) | Mouse | Ascites | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P68133 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
SIZE: 377 amino acids; 42051 Da
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
DISEASE: ... see more »
SIZE: 377 amino acids; 42051 Da
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
DISEASE: ... see more »
Entrez Gene Number:
Entrez Gene Summary
Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Description:
Anti-Actin, clone C4
Background Information:
Actin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42 kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific (Otey, 1986; Lessard, 1988).
Clone:
C4
Specificity:
MAB1501 is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertebrate actin (Lessard, 1988). The epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70. Clone C4 reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts (Lessard, 1983). Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
Key Applications:
- ELISA
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
Application Notes:
Indirect immunofluorescence at 1:100:
Tissue culture cells -- fix with formaldehyde, treat with methanol or acetone.
Glycerinated myofibrils -- fix fibers with formaldehyde, treat with cold methanol. Stains I-bands intensely and stress fibers in human fibroblasts.
Cryostat sections (6 µm) -- quick frozen in isopentane, slides treated with gelatin and formaldehyde.
Immunoblots: 1:100-1:1,000 (Otey, 1987):On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43 kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha-actin found in skeletal, cardiac, and arterial muscle.
Iodination (Lessard, 1979).
Solid phase binding assay: 1:800-1:1,000.
ELISA: strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.
Optimal working dilutions must be determined by end user.
Tissue culture cells -- fix with formaldehyde, treat with methanol or acetone.
Glycerinated myofibrils -- fix fibers with formaldehyde, treat with cold methanol. Stains I-bands intensely and stress fibers in human fibroblasts.
Cryostat sections (6 µm) -- quick frozen in isopentane, slides treated with gelatin and formaldehyde.
Immunoblots: 1:100-1:1,000 (Otey, 1987):On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43 kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha-actin found in skeletal, cardiac, and arterial muscle.
Iodination (Lessard, 1979).
Solid phase binding assay: 1:800-1:1,000.
ELISA: strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.
Optimal working dilutions must be determined by end user.
Species Reactivity:
All
Isotype:
IgG1κ
Immunogen:
Purified chicken gizzard actin (Lessard, 1988).
Epitope:
near a.a. 50-70
Molecular Weight:
43 kDa
Control:
HeLa whole cell lysate
Presentation:
Liquid, with 0.01% sodium azide.
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- ACTA1
- MPFD
- ASMA
- NEM1
- CFTDM
- ACTA
- CFTD
- NEM2
- Alpha-actin-1
- CFTD1
- NEM3
Antibody Category:
Cell Structure
Antibody Sub-Category:
Cytoskeleton
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Concentration:
Variable
Format Code:
Asc
Trade Name:
Chemicon (Millipore)
Purification Method:
UnPurified
Format:
Ascites
Antibody Type:
Monoclonal Antibody
Host:
Mouse