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Frozen section of tonsils showing endothelial staining (Catalog Number CBL468).
Anti-PECAM-1, domains 3-6 of human PECAM-1, clone HC1/6
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IP, IH, IH(P) | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P16284 # This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.
SIZE: 738 amino acids; 82536 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the ... see more »
SIZE: 738 amino acids; 82536 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the ... see more »
Entrez Gene Number:
Description:
Anti-PECAM-1, domains 3-6 of human PECAM-1, clone HC1/6
Alternate Names:
CD31
Clone:
HC1/6
Specificity:
Reacts with a 130-140 kDa transmembranous glycoprotein that is expressed widely amongst cells of the vascular compartment. CD31 is expressed on platelets, cultured endothelial cells, monocytes, normal tissue macrophages in tonsil, lung and kidney as well as in skin biopsies from conditions such as sarcoidosis and lepromatous leprosy.
FUSION PARTNER: P3-x63Ag8.653 myeloma cell line
FUSION PARTNER: P3-x63Ag8.653 myeloma cell line
Key Applications:
- Flow Cytometry
- Immunoprecipitation
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
Application Notes:
Studies of the role of CD31 in leucocyte migration into inflammatory sites and in the process of angiogenesis
This antibody will stain frozen tissue sections
Suitable for immunoprecipitation studies
Suitable for flow cytometry either indirectly or directly when labelled with FITC or R-PE
Suitable for use on formalin fixed paraffin embedded sections after protease pre-treatment.
Optimal working dilutions must be determined by the end user.
This antibody will stain frozen tissue sections
Suitable for immunoprecipitation studies
Suitable for flow cytometry either indirectly or directly when labelled with FITC or R-PE
Suitable for use on formalin fixed paraffin embedded sections after protease pre-treatment.
Optimal working dilutions must be determined by the end user.
Species Reactivity:
Human
Isotype:
IgG1
Immunogen:
PMA - treated U937 cells
Epitope:
domains 3-6 of human PECAM-1
Control:
Activated microglial cells
Presentation:
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Storage Conditions:
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.
Gene Symbol:
- PECAM1
- EndoCAM
- GPIIA'
- PECAM-1
- CD31
- CD31/EndoCAM
Antibody Category:
ECM & Adhesion
Antibody Sub-Category:
Adhesion (CAMs)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Concentration:
0.1 mg/mL
Format Code:
Pur
Trade Name:
Chemicon (Millipore)
Purification Method:
Protein A Purified
Format:
Purified
Antibody Type:
Monoclonal Antibody
Host:
Mouse