Anti-Actin, smooth muscle, clone ASM-1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, Eq, H, M, R | IF, WB, IH, IH(P) | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P62736 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
SIZE: 377 amino acids; 42009 Da
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
SIMILARITY: SwissProt: P627 ... see more »
SIZE: 377 amino acids; 42009 Da
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
SIMILARITY: SwissProt: P627 ... see more »
Entrez Gene Number:
Entrez Gene Summary
Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Description:
Anti-Actin, smooth muscle, clone ASM-1
Background Information:
ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. This antibody reacts with many types of smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues, and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland. Myogenic soft tissues detected include leiomyosarcomas, leiomyomas, and certain stromal cells surrounding infiltrating ductal carcinoma of the breast.
Clone:
ASM-1
Specificity:
Specific for the alpha-smooth-muscle isoform of actin.
Key Applications:
- Immunofluorescence
- Western Blotting
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
Application Notes:
Immunohistochemistry: 1:2000, Frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded section
Western blotting
Immunofluorescence
Optimal working dilutions must be determined by the end user.
Western blotting
Immunofluorescence
Optimal working dilutions must be determined by the end user.
Species Reactivity:
- Bovine
- Chicken
- Horse
- Human
- Mouse
- Rat
Isotype:
IgG2a
Immunogen:
Synthetic peptide corresponding to the N-terminal amino acids of the alpha-smooth muscle isoform of actin coupled to KLH.
Epitope:
N-terminus
Molecular Weight:
43 kDa
Control:
Positive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.
Presentation:
Protein A Purified immunoglobulin in PBS containing 0.09% sodium azide.
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- ACTA2
- Alpha-actin-2
- ACTSA
Antibody Category:
Cell Structure
Antibody Sub-Category:
Cytoskeleton
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Concentration:
1 mg/mL
Format Code:
Pur
Trade Name:
Chemicon (Millipore)
Purification Method:
Protein A Purified
Format:
Purified
Antibody Type:
Monoclonal Antibody
Host:
Mouse