Goat anti-Human IgG, Fc, Alkaline Phosphatase conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, WB | Goat | Alkaline Phosphatase | Polyclonal Antibody |
Description:
Goat anti-Human IgG, Fc, Alkaline Phosphatase conjugate
Background Information:
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Specificity:
Based on immunoelectrophoresis, the antibody reacts with the heavy chain of human IgG but not with the light chains of most human immunoglobulins.. No antibody was detected against normal human IgM or IgA, or against non-immunoglobulin serum proteins, but the antibody may cross-react with immunoglobulins from other species.
Key Applications:
- ELISA
- Western Blotting
Application Notes:
EIA and Western blots: 1:5,000-1:50,000.
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Species Reactivity:
Human
Presentation:
Lyophilized. Buffer = 0.01 M Tirs HCl, 0.25 M NaCl, pH 8.0 with
15 mg/mL BSA and 0.05% sodium azide.
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.
15 mg/mL BSA and 0.05% sodium azide.
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.
Storage Conditions:
Maintain lyophilized product at 2–8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade for better) to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING:
For research use only; not for use as a diagnostic.
WARNING:
For research use only; not for use as a diagnostic.
Antibody Category:
Secondary & Control Antibodies
Antibody Sub-Category:
Fragment Specific Secondary Antibodies
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Purification Method:
immunoaffinity Purified
Format:
Alkaline Phosphatase
Product Overview:
Alkaline Phosphatase-conjugated Affinity Purified Goat anti-Human IgG
Format Code:
Alkaline Phosphatase
Antibody Type:
Polyclonal Antibody
Host:
Goat