Ordering Information
Product Images
Rabbit anti-NG2 (Catalog Number AB5320) staining of adult rat cortex. Photo shows oligodendrocyte precursor cell.
Anti-NG2 Chondroitin Sulfate Proteoglycan
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Mk, R | ELISA, IP, WB, IC, IH | Rabbit | Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May reg
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Entrez Gene Number:
Entrez Gene Summary
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Description:
Anti-NG2 Chondroitin Sulfate Proteoglycan
Background Information:
NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.
Specificity:
Recognizes NG2 Chondroitin Sulfate Proteoglycan. AB5320 identifies both the intact proteoglycan and the core protein by Western blot and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.
Key Applications:
- ELISA
- Immunoprecipitation
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry
Application Notes:
Western blot: 1:600-1:1,500. The core NG2 glycoprotein is 300kDa.
Immunocytochemistry: 1:150-1:600.
Immunohistochemistry: 1:200 on frozen sections of embryonic mouse brain using an Alexa Fluor conjugated secondary antibody. Care should be taken to avoid overfixing tissue sections. A short (5-10 minute) fix in 2-4% PFA is recommended.
Immunoprecipitation: 2 µg/mL.
ELISA: 1:1,500-1:3,000.
Optimal working dilutions and protocols must be determined by end user.
Immunocytochemistry: 1:150-1:600.
Immunohistochemistry: 1:200 on frozen sections of embryonic mouse brain using an Alexa Fluor conjugated secondary antibody. Care should be taken to avoid overfixing tissue sections. A short (5-10 minute) fix in 2-4% PFA is recommended.
Immunoprecipitation: 2 µg/mL.
ELISA: 1:1,500-1:3,000.
Optimal working dilutions and protocols must be determined by end user.
Species Reactivity:
- Human
- Mouse
- Monkey
- Rat
Immunogen:
Immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat.
Molecular Weight:
270-300 kDa
Control:
Human melanoma, glioma and proliferating brain endothelial cells, rat B49 glial cell extracts or embryonic mouse brain tissue
Presentation:
Purified immunoglobulin. Antibody Purified by protein A, then placed over a fibronectin affinity column. Liquid in PBS containing 0.02% azide.
Storage Conditions:
Maintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- CSPG4
- MSK16
- MCSP
- NG2
- MCSPG
- MEL-CSPG
- HMW-MAA
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Concentration:
1 mg/mL
Format Code:
Pur
Trade Name:
Chemicon (Millipore)
Purification Method:
Protein A Purified, then immmunodepletion
Format:
Purified
Antibody Type:
Polyclonal Antibody
Host:
Rabbit