Anti-Angiopoietin-1, N-terminus
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q15389 # Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
SIZE: 498 amino acids; 57513 Da
SUBCELLUL ... see more »
SIZE: 498 amino acids; 57513 Da
SUBCELLUL ... see more »
Entrez Gene Number:
Entrez Gene Summary
Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel
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Description:
Anti-Angiopoietin-1, N-terminus
Background Information:
The remodelling of the primitive vasculature in mammals to form the mature cardiovascular system includes a number of factors directing endothelial cell development. Angiopoietin-1 (mouse and human Ang-1; 498aa with 98% identity) is an angiogenic secreted protein that interacts with the endothelial specific Tie-2 receptor. It is primarily expressed in developing endothelial cells. During embryonic dvevelopment, Ang-1 binds and induces tyrosine phosphorylation of Tie-2. Ang-1 appears to play a crucial role in mediating matrix and mesenchyme. It mediates blood vessel maturation and stability. Ang-1 may play a critical role in heart development. Ang-1 deficient mice mimic the phenotype exhibited by animals deficient in Tie-2. A homolog of Ang-1, termed Angiopoietin-2 (mouse and human Ang-2, 496aa with 85% identity) may act as an antagonist for Ang-1 and Tie-2. Ang-1 and Ang-2 have ~60% sequence homology.
Alternate Names:
Ang-1
Specificity:
Recognizes mouse Angiopoietin-1 (Ang-1). The immunogenic peptide sequence is 90% (18/20 aa) conserved in human Ang-1, 95% I pig and bovine, and has no significant sequence homology with Ang-2. Antibody crossreactivity in other species is not established.
Key Applications:
- ELISA
- Western Blotting
Application Notes:
Western blot: 1-10 ug/mL using ECL. Expected size of Ang-1 is ~55kDa.
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide per well (Catalog Number AG635).
Immunohistochemistry: Not tested. It is recommended that the antibody be used at 2-20 ug/ml in formaldehyde fixed tissue.
Optimal working dilutions must be determined by end user.
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide per well (Catalog Number AG635).
Immunohistochemistry: Not tested. It is recommended that the antibody be used at 2-20 ug/ml in formaldehyde fixed tissue.
Optimal working dilutions must be determined by end user.
Species Reactivity:
Mouse
Immunogen:
20 amino acid peptide sequence near the amino-terminus of mouse Ang-1.
Epitope:
N-terminus
Molecular Weight:
55 kDa
Control:
Fetal heart
Presentation:
Affinity Purified immunoglobulin. Liquid in Tris, pH 7.4, containing 0.1% BSA as stabilizer and 0.02% sodium azide as preservative.
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- ANGPT1
- ANG1
- ANG-1
- AGP1
- KIAA0003
- Ang1
- AGPT
Antibody Category:
ECM & Adhesion
Antibody Sub-Category:
ECM Proteins
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Concentration:
0.5 mg/mL
Format Code:
APur
Trade Name:
Chemicon (Millipore)
Purification Method:
Immunoaffinity Purified
Format:
Affinity Purified
Antibody Type:
Polyclonal Antibody
Host:
Rabbit