Ordering Information
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Western Blot Analysis:
Cat. # AB2219 can detect Aquaporin 1 at 0.5 µg/mL in adult rat heart Lysate.
Anti-Aquaporin 1
Recommended Replacement for: Cat. # AB3065| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ELISA, WB, IH | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P29972 # Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
SIZE: 269 amino acids; 28526 Da
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in a number of tissues including erythrocytes, renal t ... see more »
SIZE: 269 amino acids; 28526 Da
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in a number of tissues including erythrocytes, renal t ... see more »
Entrez Gene Number:
Entrez Gene Summary
Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement.
Description:
Anti-Aquaporin 1
Replaces:
Cat. # AB3065
Background Information:
Aquaporin 1 is a 28 kDa integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Alternate Names:
AQP1; CHIP; CHIP28
Specificity:
Recognizes Aquaporin 1.
Key Applications:
- ELISA
- Western Blotting
- Immunohistochemistry
Application Notes:
Immunohistochemistry: 2-10 µg/mL on paraformaldehyde fixed tissue sections.
Western Blotting: 1-10 µg/mL using Chemiluminescence technique. Immunoblots reveal two bands, a lower 28 kDa band corresponding to unglycosylated form and an upper high molecular 35-60 kDa glycosylated component (Denker et al. 1988; Smith & Agre 1991) We recommend the use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
ELISA: 0.5-1.0 µg/mL using 1 µg/mL control peptide (Catalog Number AG307)/well.
Optimal working dilutions must be determined by end user.
Western Blotting: 1-10 µg/mL using Chemiluminescence technique. Immunoblots reveal two bands, a lower 28 kDa band corresponding to unglycosylated form and an upper high molecular 35-60 kDa glycosylated component (Denker et al. 1988; Smith & Agre 1991) We recommend the use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
ELISA: 0.5-1.0 µg/mL using 1 µg/mL control peptide (Catalog Number AG307)/well.
Optimal working dilutions must be determined by end user.
Species Reactivity:
Rat
Immunogen:
A 19 aa synthetic peptide from the cytosolic carboxyl terminal domain of the AQP1 (Lanahan et al. 1992; Deen et al. 1992; Moon et al. 1993; Patil et al. 1994) conjugated to KLH. This peptide contains most of the epitopes recognized by polyclonal antibodies against the whole protein (Smith & Agre 1991). The immunogen peptide is absolutely conserved in AQP1 from human, ovine, bovine, rat, and mouse.
This peptide is available for blocking experiments (catalog number: AG307).
This peptide is available for blocking experiments (catalog number: AG307).
Molecular Weight:
28 kDa
Control:
Routinely tested on adult rat heart lysate
Presentation:
Purified in PBS with 0.05% NaN3
Storage Conditions:
Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
Gene Symbol:
- AQP1
- Aquaporin-CHIP
- MGC26324
- AQP-CHIP
- CO
- Aquaporin-1
- CHIP28
- AQP-1
Antibody Category:
Neuroscience
Antibody Sub-Category:
Ion Channels & Transporters
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Format Code:
APur
Trade Name:
Chemicon (Millipore)
Purification Method:
Immunoaffinity Purified
Format:
Affinity Purified
Antibody Type:
Polyclonal Antibody
Host:
Rabbit