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Rabbit anti-Osteonectin (Catalog Number AB1858) staining of fetal calvaria bone section.
Rabbit anti-Osteonectin (Catalog Number AB1858) staining of fetal calvaria bone section.
Anti-Osteonectin
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, IH(P) | Rabbit | Serum | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P09486 # Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
SIZE: 303 amino acids; 34632 Da
SUBCELLULAR LOCATION: Secreted, extracel ... see more »
SIZE: 303 amino acids; 34632 Da
SUBCELLULAR LOCATION: Secreted, extracel ... see more »
Entrez Gene Number:
Entrez Gene Summary
Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM) (Bradshaw et al., 2003 [PubMed 12721366]).[supplied by OMIM]
Description:
Anti-Osteonectin
Background Information:
Osteonectin is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a glycoprotein with a molecular weight of 43 kDa and has calcium-binding properties.
Alternate Names:
SPARC
Specificity:
Human Osteonectin (ON).
Key Applications:
- ELISA
- Immunohistochemistry (Paraffin)
Application Notes:
ELISA (1:5,000)
Immunohistochemistry on paraffin-embedded tissue sections (1:1,000)
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on paraffin-embedded tissue sections (1:1,000)
Optimal working dilutions must be determined by the end user.
Species Reactivity:
Human
Immunogen:
recombinant osteonectin expressed in human embryonal kidney line HEK-293.
Presentation:
Rabbit serum. Lyophilized. Reconstitute with 100 µL of sterile distillled water.
Storage Conditions:
Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Gene Symbol:
- SPARC
- Osteonectin
- BM-40
- ON
Antibody Category:
Cell Structure
Antibody Sub-Category:
Osteobiology
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Trade Name:
Chemicon (Millipore)
Format:
Serum
Antibody Type:
Polyclonal Antibody
Host:
Rabbit