Anti-ALG2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IC | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q9H553 # Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)- dolichol diphosphate.
SIZE: 416 amino acids; 47092 Da
DISEASE: SwissProt: Q9H553 # Defects in ALG2 are the cause of congenital disorder of glycosylation type 1I (CDG1I) [MIM:607906]. CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated ... see more »
SIZE: 416 amino acids; 47092 Da
DISEASE: SwissProt: Q9H553 # Defects in ALG2 are the cause of congenital disorder of glycosylation type 1I (CDG1I) [MIM:607906]. CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii).
Description:
Anti-ALG2
Background Information:
The Apoptosis-linked gene 2 (ALG2) gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. ALG2 is required for cell death induced by different apoptotic stimuli. ALG2 protein is expressed in normal brains, and expression is increased in ischemic brains after transient focal cerebral ischemia.
Specificity:
ALG2 (Alpha-1,3-mannosyltransferase). By Western blot the antibody reacts with a band at ~ 47 kDa on human HepG2 cell lysate. An additional faint band at ~ 42-45 kDa may also be seen depending on sample and antibody concentration used.
Key Applications:
- Western Blotting
- Immunocytochemistry
Application Notes:
Western Blot Analysis: 0.5 µg/mL of Cat. #AB15854 was used to detect ALG2 in 10 µg of human HepG2 tissue lysate
Immunocytochemistry: 2 µg/mL of Cat. AB15854 was used to detect ALG2 in Human HepG2 cells.
Immunocytochemistry: 2 µg/mL of Cat. AB15854 was used to detect ALG2 in Human HepG2 cells.
Species Reactivity:
Human
Immunogen:
Synthetic peptide from human ALG2.
Epitope:
C-Terminus
Molecular Weight:
47 kDa
Control:
Tested on human HepG2 tissue lysate
Presentation:
Purified Antibody in PBS with 0.1% NaN3
Storage Conditions:
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Gene Symbol:
- ALG2
- CDGIi
- ORFNames=UNQ666/PRO1298
- CDGII
- 1lcNAc
- hALPG2
- OTTHUMP00000021785
- FLJ14511
- EC 2.4.1.-
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neurodegenerative Diseases
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Purification Method:
Immuno Affinity Purified
Format:
Affinity Purified
Antibody Type:
Polyclonal Antibody
Host:
Rabbit