Human Plasma Fibronectin Purified Protein
Description:
Human Plasma Fibronectin Purified Protein
Trade Name:
Chemicon (Millipore)
Product Overview:
Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.
Key Applications:
Cell Culture
Application Notes:
Immunochemical standard
Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts
Suggested Procedure for Coating Cell Cultureware
1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (µg/mL) of HFN. Recommended amount is 2-10 µg/cm2.
2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.
3.Introduce the proper CO2 atmosphere, if required.
4.Add the calculated amount of HFN to each culture vessel.
5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.
6.Remove residual balanced salt solution before proceeding with standard cell culture procedures
Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts
Suggested Procedure for Coating Cell Cultureware
1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (µg/mL) of HFN. Recommended amount is 2-10 µg/cm2.
2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.
3.Introduce the proper CO2 atmosphere, if required.
4.Add the calculated amount of HFN to each culture vessel.
5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.
6.Remove residual balanced salt solution before proceeding with standard cell culture procedures
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell Culture Reagent Type:
- Stem Cell Reagents
- Attachment Factors
Descriptive Text:
Fibronectin (FN) exists in 3 forms: plasma FN, a soluble dimeric form that becomes incororated into the Fibrin meshwork of clots; cell-surface FN, oligomers transiently attached to the cell surface; and matrix FN, highly insoluble fibrils in the extracellular matrix. The 110 kDa cell-binding fragment of FN serves as a substrate for cells that express the α5β1 Integrin receptor and other Integrin receptors that recognize the Arg-Gly-Asp (RGD) sequence. It does not contain the Hep-2/CS1 region that is recognized by α4β1 Integrin.
Concentration:
1 mg/mL
Stem Cell Workflow Stage:
- Maintenance & Expansion
- General Cell Culture
- Differentiation
Gene Symbol:
- FN1
- DKFZp686O13149
- DKFZp686H0342
- DKFZp686I1370
- MSF
- FN
- CIG
- DKFZp686F10164
- LETS
- FINC
Protein Type:
Purified Protein
Presentation:
Liquid in 150 mM NaCl, 10 mM sodium phosphate, pH 7.5, sterile filtered, containing no preservatives.
Stem Cell Type:
- Human Embryonic Stem Cells
- Mesenchymal Stem Cells
- Neural Stem Cells
- Hematopoietic Stem Cells
- Epithelial Progenitor Cells
- Pancreatic Stem Cells
Purity:
Approximately 95%, as determined by SDS-PAGE. A double band of 220 kDa is present under reduced conditions. HFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose.
Protein or Enzyme Type:
Extracellular Matrix Proteins
Storage Conditions:
Maintain at 2-8°C for up to 6 months from date of receipt. Do not freeze.
Species:
Human
Source:
Human donor plasma.