cGMP HTS Immunoassay Kit (Chemiluminescent)

cGMP HTS Immunoassay Kit (Chemiluminescent)

Upstate (Millipore)

Upstate®'s cGMP HTS Immunoassay kit is a competitive immunoassay for highly sensitive and rapid chemiluminescent quantitation of cyclic GMP (cGMP, Guanosine 3', 5'-cyclic monophosphate) from extracts, lysates or supernatants of cells of any species. The kit comprises a specific anti-cGMP antibody which recognizes all species, an alkaline phosphatase (AP)-labeled cGMP conjugate, two 96 well microplates pre-coated with ananti-Rabbit antibody, cGMP standard, and an Alkaline Phosphate chemiluminescent substrate. This kit contains antibody coated plates and all reagents required to perform 192 assays.

ELISA

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

cyclic GMP ELISA

Cyclic GMP (cGMP, Guanosine 3’, 5’-cyclic monophosphate) is a nucleotide which acts as a key second messenger in multiple signal transduction pathways. cGMP is synthesized from guanosine triphosphate (GTP) by the action of guanylate cyclase, degraded by cyclic nucleotide phosphodiesterases, which hydrolyze cGMP into 5’-GMP. Levels of cGMP are increased by the action of certain hormones, such as acetylcholine, insulin and oxytocin, as well as by chemicals such as serotonin and histamine. cGMP can also be elevated by agents which stimulate guanylate cyclase, such as nitroprusside, nitroglycerin, sodium nitrate and nitric oxide. cGMP has become an important target for drug discovery because it regulates many cellular functions, including ion channel conductance, glycogenolysis and cellular apoptosis. Significantly, it also relaxes smooth muscle tissues. In blood vessels, relaxation of vascular smooth muscles leads to vasodilation and increased blood flow.

Cross-Reactivity
cGMP 100%,
cAMP <0.1%,
GTP <0.01%,
GMP <0.01%,
CTP <0.01%,
AMP <0.01%,
ADP <0.01%,
ATP <0.01%.

1. Multi-channel or repeating pipettes
2. Pipettors & tips capable of accurately measuring 10-1000 µL
3. Plate shaker (optional)
4. Graduated serological pipettes
5. Luminometer capable of reading 96-well microplates.
6. Polystyrene test tubes for standard and sample dilutions
7. Mechanical vortex
8. Containers for dilutions

• 1. White 96-Well anti-Rabbit Coated Plate: (Part No. 2004070) Two 96-well immuno-plates pre-coated with anti-Rabbit polyclonal antibody, sealed in a foil pouch.
• 2. Rabbit anti-cGMP polyclonal Antibody: (Part No. 2004761) One 25 µL vial.
• 3. cGMP Standard: (Part No. 2004762) One 2 mL bottle
• 4. Alkaline Phosphatase cGMP Tracer Conjugate: (Part No. 2004760) One 20 µL vial
• 5. 2x cGMP Assay Diluent: (Part No. 2004811) One 25 mL bottle.
• 6. 5x Wash Buffer: (Part No. 2004075) One 60 mL bottle.
• 7. Lysis Buffer: (Part No. 2004076) One 125 mL bottle.
• 8. 6x Alkaline Phosphatase Substrate: (Part No. 2004077) One 5 mL bottle.

cGMP

Routinely evaluated by ELISA.

Chemiluminescent

Detection Kits

1 year at 4°C from date of shipment