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The provided cAMP standard containing 1 mM cAMP (5000 pmol/µl) was used in this experiment. Seven serial 1:10 dilutions were prepared in Lysis buffer ...
cAMP HTS Immunoassay Kit (Chemiluminescent)
Description:
cAMP HTS Immunoassay Kit (Chemiluminescent)
Trade Name:
Upstate (Millipore)
Key Applications:
ELISA
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Cyclic AMP (cAMP, adenosine 3´, 5´-cyclic monophosphate) is a nucleotide which acts as a key second messenger in multiple signal transduction pathways. cAMP regulates many cellular functions, such as metabolism, cell growth and differentiation, gene transcription, ion transport and ion channel function. It is synthesized from ATP by the action of adenylate cyclase, and is inactivated by hydrolysis to 5´-AMP by the actions of phosphodiesterases. cAMP is the downstream mediator of G proteins which activate adenylate cyclase upon ligand binding. Many different drugs, neurotransmitters and hormones exert their cellular effects by modulating adenylate cyclase activity and thus raising or lowering intracellular cAMP concentrations.
Packaging:
Kit capacity: 192 wells
Components:
- Two White 96-well anti-Rabbit Coated Plate
- Rabbit anti-cAMP antibody
- cAMP standard
- cAMP Alkaline Phosphatase Conjugate Tracer
- 2X Assay Diluent
- 5X Wash Buffer
- Lysis Buffer
- 4x Alkaline Phosphatase Substrate
Kit Type:
Detection Kits
Analytes Available:
cAMP
Quality Assurance:
Routinely evaluated by ELISA.
Detection Methods:
Chemiluminescent
Kit or Assay Type:
Detection Kits