Anti-Active-β-Catenin (anti-ABC), clone 8E7
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as α-catenin and β-catenin. α-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins.
When β-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in β-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When β-catenin is not associated with cadherins and α-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC).
Recent evidence suggests that β-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
8E7
Recognizes active form of β-catenin, dephosphorylated on Ser37 or Thr41.
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Western Blotting
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Immunocytochemistry
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Immunohistochemistry
IgG1κ
Fusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag
The epitope corresponds to amino acid residues 36-44 (HSGATTTAP) of human β-catenin.
92 kDa
Positive Control Included: Non-Stim A431 Cell Lysate (12-301). Other controls: HeLa whole cell lysate or MCF7 whole cell lysate
Immunoaffinity Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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CTNNB1
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CTNNB
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FLJ25606
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Beta-catenin
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PRO2286
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FLJ37923
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DKFZp686D02253
routinely evaluated by immunoblot on RIPA lysates from A431 cells
Nuclear Function
Transcription Factors
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Pur
Upstate (Millipore)
Protein G Purified
Purified
Monoclonal Antibody
Mouse
