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Flow Cytometry: Flow cytometry analysis using anti-phosphoserine, clone 4A4. Cells were either untreated (shaded, green) or treated (unshaded) with Ca ...
Immunofluorecence Microscopy:
Confocal immunofluorescence image of insulin-treated 293 cells labeled with Anti-phosphoserine, clone 4A4 (green) a ...
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Anti-Phosphoserine, clone 4A4
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt | WB, IF, FC, IH(P), ELISA | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Phosphoserine, clone 4A4
Background Information:
The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (greater than 95%).
Clone:
4A4
Specificity:
Serine-phosphorylated proteins from all species
Key Applications:
- Western Blotting
- Immunofluorescence
- Flow Cytometry
- Immunohistochemistry (Paraffin)
- ELISA
Species Reactivity:
Vertebrates
Isotype:
IgG1
Immunogen:
Phosphoserine coupled to KLH
Modifications:
Phosphorylation
Molecular Weight:
Dependent upon the molecular weight of the serine phosphorylated protein being detected.
Control:
Included Positive Control: Cat.# 12-628, Calyculin A/Okadaic-treated A431 cell lysate is provided as a free positive control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20ºC. The lysate is stable for 6 months at -20ºC.
Presentation:
100 µL of PBS with 0.05% sodium azide with 30% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
Quality Assurance:
Routinely evaluated by immunoblot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.
Antibody Category:
Cell Signaling
Antibody Sub-Category:
General Post-translation Modification
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Trade Name:
Upstate (Millipore)
Purification Method:
Protein G-Sepharose chromatography
Format:
Purified
Antibody Type:
Monoclonal Antibody
Host:
Mouse