Purification
This section covers the series of operations immediately following the cell culture, lysate or refold clarification process. The first of these unit operations is a capture where the product protein is typically bound to an affinity chromatography resin and then recovered by elution with a buffer solution. Once recovered, the protein solution is further purified and concentrated through filtration or additional chromatography steps until the final solution is ready for final fill operations.
The primary goals for successful protein processing are:
- High product purity
- High product quality
- Robustness
- High product yield
- Economical
Ultrafiltration (Tangential Flow Filtration) steps are used to reduce batch volumes and exchange buffers for efficient column operation or final formulation. Sequential capture, purification and polishing columns remove impurities to achieve specified product purity with high product recovery. Efficient process design maximizes the difference between product and impurity binding.
Our integrated purification solutions, which include media, filters, columns, systems, services and expertise, enable you to separate and purify a broad range of Mabs, proteins, vaccines, and other bio-engineered products from methods development to pilot and large-scale manufacturing.
PRODUCT OVERVIEW
Learn more about Separation and Purification Technologies from our Product Catalogue.
Technical Library
- A Hands-On Guide to Ultrafiltration/Diafiltration Optimization using Pellicon Cassettes
- Increase Product Yield in Your UF/DF Process
- Pellicon 2 Cleaning Effectiveness
Product News & Releases
- Millipore Introduces Solvex™ Solvent Filters - New cartridge filters ideal for sterile manufacture of APIs
- Millipore Introduces the Mobius® MIX500 Disposable Mixing System - Increased process speed and reduced risk now in a 500L size
- Millipore Expands Its NovAseptic® Mixer Family - The new GMP30000 mixer is ideal for effective mixing in large volumes
- Millipore Introduces New Service Offer for Evaluating Mixing Solutions
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